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By Herbert K. Weiss

This document includes en research of wartime files of assaults by way of German warring parties on US Bombers, and of strive against among US and German warring parties.

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Snyder PA (Ed) pp. 81-\05, American Chemical Society, Washington, DC (1996). Black GE, Snyder AP, Heroux KS. Chemotaxonomic differentiation between the Bacillus cereus group and Bacillus subtilis by phospholipid extracts analyzed with electrospray ionization tandem mass spectrometry. J Microbiol Meth 28: 187-199 (1997). Blackstock WP, Weir MP. Proteomics: quantitative and physical mapping of cellular proteins. Trends Biotechnol17: 121-127 (1999). Blagoev B, Pandey A. Microarrays go live - new prospects for proteomics.

However, metabolites may require derivatization to generate volatile analytes prior to GC separation. Although efficient derivatization methods are available sample throughput can be adversely affected. i. Metabolomics Metabolite profiling Figure 3. Strategies for metabolomic analysis. DIESI: direct-infusion ESI (refer to text for other abbreviations). Another technique that can provide valuable information on metabolomes is nuclear magnetic resonance (NMR) spectroscopy. It is based on the fact that nuclei such as 'H, 13C and 31p can exist at different energy levels in a strong magnetic field, as they possess nuclear spin.

However, even though the constituents of the metabolic complement, Le. metabolites, can be accurately quantified using standard analytical techniques, relatively fewer strategies have been developed for more comprehensive holistic profiling. Although trends are changing a majority of reported metabolic investigations have concentrated on assessing only a portion of the metabolome. MS can play a useful role in a more comprehensive metabolome profiling, but will have to be coupled with prefractionation and clean-up steps that may have to be multiplexed for highthroughput analysis.

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