By ACI Committee 363
High-strength concrete (HSC) has emerged as a plausible fabric to exploit as a substitute to standard normal-strength concrete in infrastructure platforms to minimize member go part, expand member span size, lessen the variety of process participants, or increase approach sustainability. This advisor deals common details at the quality controls and trying out of HSC. thoughts are in accordance with the present kingdom of information won from world wide experimental learn, analytical paintings, and box purposes of HSC platforms utilized in concrete constructions.
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Extra resources for ACI 363.2R-11 - Guide to Quality Control and Assurance of High-Strength Concrete
An interesting finding (Mann, 1972) was the dependence on 3 M potassium chloride for this effect. Thus, incubation of the intact cells in isotonic buffer without 3 M potassium chloride, or of isolated membranes with or without cell sap in the absence of 3 M potassium chloride, apparently leads to very little solubilisation of antigenically active material. Similarly, Fairbanks et al. (1971) have shown that proteolysis of isolated red cell membranes by endogenous membrane-bound proteases is accentuated in high-salt solutions under neutral or slightly alkaline conditions.
1967; Allan and Crumpton, 1971b), the use of detergents known not to perturb native protein conformation significantly is obviously advisable in many instances. However, it should be mentioned that sodium dodecyl sarcosinate-Tris has been used successfully to solubilise, in active form, glycoprotein enzymes from mouse liver plasma membranes (Evans and Gurd, 1973). SEPARATION AND PURIFICATION METHODS In this section, various fractionation schemes that have been applied to solubilised mixtures of membrane proteins are considered.
In the ultracentrifuge, two major fractions were found with sedimentation coefficients of 5S and 10S and a small amount of larger particles. The material, freed from butanol by dialysis, was eluted by chromatography on Sephadex G-200 in aqueous buffer as a single broad peak of average molecular weight 300 000, a result consistent with the sedimentation coefficients. The chromatographic system does not, however, separate the two components detected by ultracentrifugation. Mention was made by Maddy (1966) that further fractionation of the solubilised protein obtained by extraction with butanol could be obtained by chromatography on DEAE-cellulose.